IFT20 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98642
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human IFT20 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for IFT20 Antibody
Western Blot: IFT20 Antibody [NBP2-98642]
Western Blot: IFT20 Antibody [NBP2-98642] - Anti-IFT20 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 17 kDaImmunohistochemistry-Paraffin: IFT20 Antibody [NBP2-98642]
Immunohistochemistry-Paraffin: IFT20 Antibody [NBP2-98642] - Staining of human IFT20 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution.Immunoprecipitation: IFT20 Antibody [NBP2-98642]
Immunoprecipitation: IFT20 Antibody [NBP2-98642] - IFT20 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate. 4 ul anti-IFT20 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-IFT20 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDaApplications for IFT20 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
5-10uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFT20
Alternate Names
hIFT20, intraflagellar transport 20 homolog (Chlamydomonas), intraflagellar transport protein 20 homolog, intraflagellar transport protein IFT20
Gene Symbol
IFT20
Additional IFT20 Products
Product Specific Notices for IFT20 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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