IFT88 Antibody (JE64-77)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32459
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE64-77
Concentration
1 mg/ml
Product Summary for IFT88 Antibody (JE64-77)
Immunogen
Synthetic peptide within Human IFT88 aa 1-50 / 824. (Uniprot: Q13099)
Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IFT88 Antibody (JE64-77)
Western Blot: IFT88 Antibody (JE64-77) [NBP3-32459]
Western Blot: IFT88 Antibody (JE64-77) [NBP3-32459] - Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (NBP3-32459) at 1/1,000 dilution.Lane 1: HEK-293 cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: Mouse testis tissue lysate (40 ug/Lane)
Lane 4: Rat testis tissue lysate (20 ug/Lane)
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32459) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for IFT88 Antibody (JE64-77)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFT88
Alternate Names
D13S1056ETPR repeat protein 10, DAF19, hTg737, intraflagellar transport 88 homolog (Chlamydomonas), intraflagellar transport protein 88 homolog, MGC26259, polaris homolog, probe hTg737 (polycystic kidney disease, autosomal recessive), Recessive polycystic kidney disease protein Tg737 homolog, tetratricopeptide repeat domain 10, Tetratricopeptide repeat protein 10, TG737Tg737, TTC10
Gene Symbol
IFT88
Additional IFT88 Products
Product Documents for IFT88 Antibody (JE64-77)
Product Specific Notices for IFT88 Antibody (JE64-77)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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