IFT88 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2475

Novus Biologicals, part of Bio-Techne
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NB100-2475
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IFT88 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KKRIDEDDFADEE corresponding to C-Terminus according to NP_006522.2.

Reactivity Notes

ICC/IF successful usage for Mouse nose and lungs is reported in scientific literature (PMID: 19470799).

Specificity

This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IFT88 Antibody

Western Blot: IFT88 Antibody [NB100-2475]

Western Blot: IFT88 Antibody [NB100-2475]

Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: IFT88 Antibody [NB100-2475]

Immunocytochemistry/ Immunofluorescence: IFT88 Antibody [NB100-2475]

Immunocytochemistry/Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: IFT88 Antibody [NB100-2475]

Flow Cytometry: IFT88 Antibody [NB100-2475]

Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for IFT88 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/uL
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 19208653). WB: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background.
Immunofluorescence: This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFT88

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

D13S1056ETPR repeat protein 10, DAF19, hTg737, intraflagellar transport 88 homolog (Chlamydomonas), intraflagellar transport protein 88 homolog, MGC26259, polaris homolog, probe hTg737 (polycystic kidney disease, autosomal recessive), Recessive polycystic kidney disease protein Tg737 homolog, tetratricopeptide repeat domain 10, Tetratricopeptide repeat protein 10, TG737Tg737, TTC10

Entrez Gene IDs

8100 (Human); 21821 (Mouse); 305918 (Rat)

Gene Symbol

IFT88

UniProt

Q13099 (Human)

Additional IFT88 Products

Product Documents for IFT88 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IFT88 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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