IFT88 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2475
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KKRIDEDDFADEE corresponding to C-Terminus according to NP_006522.2.
Reactivity Notes
ICC/IF successful usage for Mouse nose and lungs is reported in scientific literature (PMID: 19470799).
Specificity
This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for IFT88 Antibody
Western Blot: IFT88 Antibody [NB100-2475]
Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: IFT88 Antibody [NB100-2475]
Immunocytochemistry/Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: IFT88 Antibody [NB100-2475]
Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for IFT88 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:128000
Western Blot
1 - 3 ug/uL
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 19208653). WB: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background.
Immunofluorescence: This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells
Immunofluorescence: This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IFT88
Alternate Names
D13S1056ETPR repeat protein 10, DAF19, hTg737, intraflagellar transport 88 homolog (Chlamydomonas), intraflagellar transport protein 88 homolog, MGC26259, polaris homolog, probe hTg737 (polycystic kidney disease, autosomal recessive), Recessive polycystic kidney disease protein Tg737 homolog, tetratricopeptide repeat domain 10, Tetratricopeptide repeat protein 10, TG737Tg737, TTC10
Gene Symbol
IFT88
UniProt
Additional IFT88 Products
Product Documents for IFT88 Antibody
Product Specific Notices for IFT88 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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