IgA Antibody (7D5F12) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61794
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Monkey
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7D5F12
Format
BSA Free
Concentration
1 mg/ml
Product Summary for IgA Antibody (7D5F12) - BSA Free
Immunogen
Purified recombinant fragment of human IgA (AA: 207-353) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IgA Antibody (7D5F12) - BSA Free
Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]
Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]
Immunocytochemistry/Immunofluorescence: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]
Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of breast cancer tissues using IghA1 mouse mAb with DAB staining.Applications for IgA Antibody (7D5F12) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:50-1:250
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA
Long Name
Immunoglobulin A
Alternate Names
Immunoglobulin A
Gene Symbol
IGHA1
Additional IgA Products
Product Documents for IgA Antibody (7D5F12) - BSA Free
Product Specific Notices for IgA Antibody (7D5F12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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