IgA Antibody (RM220) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62011B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Western Blot

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM220

Concentration

1 mg/ml

View all IgA Antibodies »

Product Summary for IgA Antibody (RM220) [Biotin]

Immunogen

Mouse IgA

Specificity

This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgA Antibody (RM220) [Biotin]

Western Blot: IgA Antibody (RM220) [Biotin] [NBP2-62011B] -

Western Blot: IgA Antibody (RM220) [Biotin] [NBP2-62011B] - Western blot of nonreduced(-) and reduced(+) mouse IgA, using 0.5 ug/mL of RM220. This antibody reacts to nonreduced IgA.
IgA Antibody (RM220) [Biotin]

ELISA: IgA Antibody (RM220) [Biotin] [NBP2-62011B] -

ELISA: IgA Antibody (RM220) [Biotin] [NBP2-62011B] - ELISA of Mouse immunoglobulins shows RM220 reacts to Mouse IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA: IgA Antibody (RM220) [Biotin] [NBP2-62011B] - A titer ELISA of Mouse IgA. The plate was coated with different amount of Mouse IgA. A serial dilution of NBP2-62011B was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

Applications for IgA Antibody (RM220) [Biotin]

Application
Recommended Usage

ELISA

0.005 ug/ml - 0.2 ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgA

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Long Name

Immunoglobulin A

Alternate Names

Immunoglobulin A

Gene Symbol

IGHA1

Additional IgA Products

Product Documents for IgA Antibody (RM220) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgA Antibody (RM220) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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