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IgA1 Antibody (RM124) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62024B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-62024B

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM124

Concentration

1 mg/ml

Product Specifications

Immunogen

Human IgA

Specificity

This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgA1 Antibody (RM124) [Biotin]

Immunohistochemistry: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - Immunohistochemistry of human tonsil tissue using Anti-Human IgA1 antibody NBP2-62024B.
ELISA: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - ELISA of human immunoglobulins shows NBP2-62024B reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with different immunoglobulins. NBP2-62024B was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.
ELISA: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - Sandwich ELISA using RM124 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains ( kappa + lambda) antibody NBP2-62024B as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

Applications for IgA1 Antibody (RM124) [Biotin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgA1

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against localinfection and to prevent access of foreign antigens to the general immunologic syste

Alternate Names

Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, uvomorulin

Gene Symbol

IGHA1

Additional IgA1 Products

Product Documents for IgA1 Antibody (RM124) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IgA1 Antibody (RM124) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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