IgA1 Antibody (RM124) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62024B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM124
Concentration
1 mg/ml
Product Specifications
Immunogen
Human IgA
Specificity
This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgA1 Antibody (RM124) [Biotin]
Immunohistochemistry: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - Immunohistochemistry of human tonsil tissue using Anti-Human IgA1 antibody NBP2-62024B.
ELISA: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - ELISA of human immunoglobulins shows NBP2-62024B reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with different immunoglobulins. NBP2-62024B was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.
ELISA: IgA1 Antibody (RM124) [Biotin] [NBP2-62024B] - Sandwich ELISA using RM124 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains ( kappa + lambda) antibody NBP2-62024B as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Applications for IgA1 Antibody (RM124) [Biotin]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgA1
Alternate Names
Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, uvomorulin
Gene Symbol
IGHA1
Additional IgA1 Products
Product Documents for IgA1 Antibody (RM124) [Biotin]
Product Specific Notices for IgA1 Antibody (RM124) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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