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IgD Antibody (RM123) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62026B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-62026B

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM123

Concentration

1 mg/ml

Product Summary for IgD Antibody (RM123) [Biotin]

Immunogen

Human IgD

Specificity

This antibody reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgD Antibody (RM123) [Biotin]

Immunohistochemistry: IgD Antibody (RM123) [Biotin] [NBP2-62026B] - Immunohistochemistry of human lymphoid tissue using Anti-Human IgD antibody NBP2-62026B.
ELISA: IgD Antibody (RM123) [Biotin] [NBP2-62026B] - ELISA of human immunoglobulins shows NBP2-62026B reacts to IgD from human plasma and IgDk from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with different immunoglobulins. NBP2-62026B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgD Antibody (RM123) [Biotin] [NBP2-62026B] - Sandwich ELISA using RM123 as the capture antibody (100 ng/well), and biotinylated anti-human light chains (kappa + lambda) antibody NBP2-62026B as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

Applications for IgD Antibody (RM123) [Biotin]

Application
Recommended Usage

Immunohistochemistry

0.5 - 2 ug/mL

Sandwich ELISA Capture

25 - 200ng/well

Sandwich ELISA Detection

0.01 - 0.1 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgD

Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Long Name

Immunoglobulin D

Alternate Names

Igh-5, Ighd, Immunoglobulin Delta

Gene Symbol

IGHD

Additional IgD Products

Product Documents for IgD Antibody (RM123) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IgD Antibody (RM123) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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