IgE Antibody (RM122) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25973
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM122
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for IgE Antibody (RM122) - Azide and BSA Free
Immunogen
Human IgE
Specificity
This antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgE Antibody (RM122) - Azide and BSA Free
Sandwich ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] -
Sandwich ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - Sandwich ELISA using RM122 as the capture antibody (25ng/well), and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] -
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] -
ELISA: IgE Antibody (RM122) - Azide and BSA Free [NBP3-25973] - ELISA of human immunoglobulins shows RM122 reacts to both IgE from human myeloma and IgE from human plasma. No cross reactivity with human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgE Antibody (RM122) - Azide and BSA Free
Application
Recommended Usage
Sandwich ELISA Capture
100 ng/well
Sandwich ELISA Detection
0.01 - 0.1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgE
Long Name
Immunoglobulin E
Alternate Names
IgE, immunoglobulin heavy constant epsilon
Gene Symbol
IGHE
Additional IgE Products
Product Documents for IgE Antibody (RM122) - Azide and BSA Free
Product Specific Notices for IgE Antibody (RM122) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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