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IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25520

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25520

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (95%), Porcine (92%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1982

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Immunogen

Recombinant fragment of human IGF-II/IGF2

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%), Sheep (87%), Bovine (87%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -

Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .
IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-II/IGF2

Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types

Long Name

Insulin-like Growth Factor II

Alternate Names

C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974

Gene Symbol

IGF2

Additional IGF-II/IGF2 Products

Product Documents for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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