IGF2BP3 Antibody (8K4H5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16205

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16205
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8K4H5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IGF2BP3 Antibodies »

Product Summary for IGF2BP3 Antibody (8K4H5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-194 of human IGF2BP3 (O00425). NKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGF2BP3 Antibody (8K4H5)

Western Blot: IGF2BP3 Antibody (8K4H5) [NBP3-16205]

Western Blot: IGF2BP3 Antibody (8K4H5) [NBP3-16205]

Western Blot: IGF2BP3 Antibody (8K4H5) [NBP3-16205] - Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (NBP3-16205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: IGF2BP3 Antibody (8K4H5) [NBP3-16205]

Immunohistochemistry-Paraffin: IGF2BP3 Antibody (8K4H5) [NBP3-16205]

Immunohistochemistry-Paraffin: IGF2BP3 Antibody (8K4H5) [NBP3-16205] - Immunohistochemistry of paraffin-embedded human oophoroma using IGF2BP3 Rabbit mAb (NBP3-16205) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for IGF2BP3 Antibody (8K4H5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGF2BP3

The protein encoded by the IGF2BP3 gene is primarily found in the nucleolus, where it can bind to the 5' UTR of theinsulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during latedevelopment. The encoded protein contains several KH domains, which are important in RNA binding and are known to beinvolved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes onother chromosomes. (provided by RefSeq)

Alternate Names

CT98, hKOC, IGF II mRNA binding protein 3, IGF2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IMP-3KH domain-containing protein overexpressed in cancer, IMP3VICKZ family member 3, insulin-like growth factor 2 mRNA binding protein 3, insulin-like growth factor 2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, KOC1DKFZp686F1078, VICKZ3cancer/testis antigen 98

Gene Symbol

IGF2BP3

Additional IGF2BP3 Products

Product Documents for IGF2BP3 Antibody (8K4H5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IGF2BP3 Antibody (8K4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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