IgG Heavy Chain Antibody (IG217 + IG266)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13949
![Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG217 + IG266) [NBP3-13949]](https://resources.bio-techne.com/images/products/IgG-Heavy-Chain-Antibody-IG217-+-IG266-Immunohistochemistry-Paraffin-NBP3-13949-img0001.jpg "Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG217 + IG266) [NBP3-13949]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG2a Kappa Clone # IG217 + IG266
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified human IgG
Localization
Cytoplasm, Cell Surface and Secreted
Specificity
This monoclonal antibody is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkins lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG2a Kappa
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14095)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for IgG Heavy Chain Antibody (IG217 + IG266)
Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG217 + IG266) [NBP3-13949]
Immunohistochemistry-Paraffin: IgG Heavy Chain Antibody (IG217 + IG266) [NBP3-13949] - Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Heavy Chain antibody (IG217 + IG266)Applications for IgG Heavy Chain Antibody (IG217 + IG266)
Application
Recommended Usage
Flow Cytometry
1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: IgG Heavy Chain
Alternate Names
Immunoglobulin G, ImmunoglobulinG
UniProt
Additional IgG Heavy Chain Products
Product Documents for IgG Heavy Chain Antibody (IG217 + IG266)
Product Specific Notices for IgG Heavy Chain Antibody (IG217 + IG266)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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