IgG1 Antibody (RM106) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62006B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM106
Concentration
1 mg/ml
Product Summary for IgG1 Antibody (RM106) [Biotin]
Immunogen
Mouse IgG
Specificity
This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG1 Antibody (RM106) [Biotin]
Western Blot: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20 ng/lane), using 0.2 ug/mL NBP2-62006B. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced form (~50 kDa).
ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - ELISA of mouse immunoglobulins shows NBP2-62006B reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins.NBP2-62006B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of NBP2-62006B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for IgG1 Antibody (RM106) [Biotin]
Application
Recommended Usage
ELISA
0.005 ug/ml-0.2 ug/ml
Western Blot
0.1 ug/ml - 0.5 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG1
Long Name
Immunoglobulin G1
Alternate Names
IgG1, Igh-4, RGD1359539, VH7183
Additional IgG1 Products
Product Documents for IgG1 Antibody (RM106) [Biotin]
Product Specific Notices for IgG1 Antibody (RM106) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...