Non-species specific IgG2a Isotype Control (IGG2a/6723)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27042
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # IGG2a/6723
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human IgG2a protein
Localization
None
Specificity
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Non-species specific IgG2a Isotype Control (IGG2a/6723)
Immunohistochemistry-Paraffin: Non-species specific IgG2a Isotype Control (IGG2a/6723) [NBP3-27042] -
Formalin-fixed, paraffin-embedded human tonsil stained with Non-species specific IgG2a Isotype Control (IGG2a/6723). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.Applications for Non-species specific IgG2a Isotype Control (IGG2a/6723)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml for 30 minutes at RT
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Non-species specific IgG2a Isotype Control (IGG2a/6723)
Product Specific Notices for Non-species specific IgG2a Isotype Control (IGG2a/6723)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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