IgJ Antibody (OTI3B3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71016
![Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]](https://resources.bio-techne.com/images/products/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71016-img0005.jpg "Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]")
![Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]](https://resources.bio-techne.com/images/products/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71016-img0002.jpg "Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]")
![Western Blot: IgJ Antibody (OTI3B3)Azide and BSA Free [NBP2-71016]](https://resources.bio-techne.com/images/products/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Western-Blot-NBP2-71016-img0001.jpg "Western Blot: IgJ Antibody (OTI3B3)Azide and BSA Free [NBP2-71016]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3B3
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human IGJ(NP_653247) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IgJ Antibody (OTI3B3) - Azide and BSA Free
Western Blot: IgJ Antibody (OTI3B3)Azide and BSA Free [NBP2-71016]
Western Blot: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IgJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IgJ.![Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]](https://resources.bio-techne.com/images/products/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71016-img0004.jpg "Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]")
Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]
Immunohistochemistry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Staining of paraffin-embedded Human lymphoma tissue using anti-IgJ mouse monoclonal antibody.![Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]](https://resources.bio-techne.com/images/products/IgJ-Antibody-OTI3B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71016-img0003.jpg "Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]")
Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016]
Flow Cytometry: IgJ Antibody (OTI3B3) - Azide and BSA Free [NBP2-71016] - Analysis of Jurkat cells, using anti-IgJ antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for IgJ Antibody (OTI3B3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgJ
Alternate Names
IGCJJCH, immunoglobulin J chain, immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mupolypeptides
Gene Symbol
JCHAIN
Additional IgJ Products
Product Documents for IgJ Antibody (OTI3B3) - Azide and BSA Free
Product Specific Notices for IgJ Antibody (OTI3B3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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