IgM Antibody (RM121)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62022
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM121
Concentration
1 mg/ml
Product Summary for IgM Antibody (RM121)
Immunogen
Human IgM
Specificity
This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgM Antibody (RM121)
ELISA: IgM Antibody (RM121) [Unconjugated] [NBP2-62022] - ELISA of human immunoglobulins shows NBP2-62022 reacts only to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with different immunoglobulins. NBP2-62022 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgM Antibody (RM121) [Unconjugated] [NBP2-62022] - A titer ELISA using NBP2-62022. The plate was coated with different amounts of human IgM. A serial dilution of NBP2-62022 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgM Antibody (RM121) [Unconjugated] [NBP2-62022] - Sandwich ELISA using NBP2-62022 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody (NBP2-77396) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Applications for IgM Antibody (RM121)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5 - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for IgM Antibody (RM121)
Product Specific Notices for IgM Antibody (RM121)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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