IGSF2/CD101 Antibody (BB27)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65269

Clone BB27 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65269
NB100-65269-0.025mg
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Conjugate
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Alexa Fluor 350
NB100-65269AF350
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Alexa Fluor 405
NB100-65269AF405
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Alexa Fluor 488
NB100-65269AF488
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Alexa Fluor 532
NB100-65269AF532
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Alexa Fluor 594
NB100-65269AF594
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Alexa Fluor 647
NB100-65269AF647
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Alexa Fluor 700
NB100-65269AF700
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Alexa Fluor 750
NB100-65269AF750
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Allophycocyanin
NB100-65269APC
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Allophycocyanin/Cy7
NB100-65269APCCY7
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Biotin
NB100-65269B
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CoraFluor 1
NB100-65269CL1
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DyLight 350
NB100-65269UV
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DyLight 405
NB100-65269V
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DyLight 488
NB100-65269G
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DyLight 550
NB100-65269R
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DyLight 594
NB100-65269DL594
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DyLight 650
NB100-65269C
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DyLight 680
NB100-65269FR
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DyLight 755
NB100-65269IR
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FITC
NB100-63317
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FITC
NB100-65269F
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HRP
NB100-65269H
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Janelia Fluor 525
NB100-65269JF525
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Janelia Fluor 549
NB100-65269JF549
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Janelia Fluor 585
NB100-65269JF585
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Janelia Fluor 635
NB100-65269JF635
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Janelia Fluor 646
NB100-65269JF646
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Janelia Fluor 669
NB100-65269JF669
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mFluor Violet 450 SE
NB100-65269MFV450
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mFluor Violet 500 SE
NB100-65269MFV500
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mFluor Violet 610 SE
NB100-65269MFV610
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PE
NB100-65269PE
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PE/Atto594
NB100-65269PEATT594
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PE/Cy5.5
NB100-65269PECY55
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PE/Cy7
NB100-65269PECY7
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PerCP
NB100-65269PCP
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BB27

Concentration

1.0 mg/ml

View all IGSF2/CD101 Antibodies »

Product Specifications

Immunogen

Human thymic clone B12

Specificity

NB100-65269 recognizes human CD101. CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. Clone BB27 has been reported to inhibit allogeneic T-cell responses (1). Removal of sodium azide is recommended prior to use in functional assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IGSF2/CD101 Antibody (BB27)

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.
Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock.

Applications for IGSF2/CD101 Antibody (BB27)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGSF2/CD101

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Gene Symbol

CD101

UniProt

Q93033 (Human)

Additional IGSF2/CD101 Products

Product Documents for IGSF2/CD101 Antibody (BB27)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IGSF2/CD101 Antibody (BB27)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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