Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10A9B6

Format

Azide Free

Concentration

1.0 mg/ml

View all IKK beta Antibodies »

Product Specifications

Immunogen

Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IKK beta Antibody (10A9B6) - Azide Free

Western Blot: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Western Blot: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Western Blot: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214]

Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214]

Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody.
Simple Western: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Simple Western: IKK beta Antibody (10A9B6)Azide Free [NBP2-33214]

Simple Western: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
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Applications for IKK beta Antibody (10A9B6) - Azide Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Gel Super Shift Assays

reported in scientific literature (PMID 19662631)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24825920)

Immunoprecipitation

2-5 ug/10^6 cells

Simple Western

1:12.5

Western Blot

2-5 ug/ml
Application Notes
An approx. 87 kDa band should be observed. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse)

Gene Symbol

IKBKB

Additional IKK beta Products

Product Documents for IKK beta Antibody (10A9B6) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IKK beta Antibody (10A9B6) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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