IKK beta Antibody (JE32-81)

Western Blot: IKK beta Antibody (JE32-81) [NBP3-32463]

Western Blot: IKK beta Antibody (JE32-81) [NBP3-32463] - Western blot analysis of IKK beta on different lysates with Rabbit anti-IKK beta antibody (NBP3-32463) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: HL-60 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HeLa cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 87 kDa
Observed band size: 87 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32463) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: IKK beta Antibody (JE32-81) [NBP3-32463]

Flow Cytometry: IKK beta Antibody (JE32-81) [NBP3-32463] - Flow cytometric analysis of THP-1 cells labeling IKK beta.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32463, 1/50) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).