IL-21 Antibody (MT216G/21.3m)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18290

Novus Biologicals, part of Bio-Techne
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NBP3-18290
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Key Product Details

Species Reactivity

Human, Non-human Primate

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MT216G/21.3m

Concentration

1 mg/ml

View all IL-21 Antibodies »

Product Specifications

Immunogen

Recombinant human IL-21

Specificity

Native and recombinant IL-21

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IL-21 Antibody (MT216G/21.3m)

Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290]

Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290]

Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.

Applications for IL-21 Antibody (MT216G/21.3m)

Application
Recommended Usage

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered PBS

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-21

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

Additional IL-21 Products

Product Documents for IL-21 Antibody (MT216G/21.3m)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-21 Antibody (MT216G/21.3m)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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