Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Direct ELISA, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # IL33305B

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL-33 Antibodies »

Product Specifications

Immunogen

Recombinant human IL-33.

Specificity

Recognizes human IL-33. Does not cross-react with mouse IL-33.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IL-33 Antibody (IL33305B) - Azide and BSA Free

Western Blot: IL-33 Antibody (IL33305B)Azide and BSA Free [NBP3-11681]

Western Blot: IL-33 Antibody (IL33305B)Azide and BSA Free [NBP3-11681]

Western Blot: IL-33 Antibody (IL33305B) [NBP3-11681] - Western blot analysis of human IL-33 using IL-33 antibody (IL33305B) at 1:2,000 dilution.1. Recombinant human IL-33 (His tagged).2. Recombinant human IL-33 (FLAG(R)-tagged).3. Recombinant mouse IL-33 (FLAG(R)-tagged).4. Other protein (His tagged, negative control).
Immunohistochemistry: IL-33 Antibody (IL33305B) - Azide and BSA Free [NBP3-11681]

Immunohistochemistry: IL-33 Antibody (IL33305B) - Azide and BSA Free [NBP3-11681]

Immunohistochemistry: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunohistochemical staining of human tissue using IL-33 antibody (IL33305B) at 1:100 dilution.A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour).B. Isotype control with IgG2a (negative control).
Immunoprecipitation: IL-33 Antibody (IL33305B) - Azide and BSA Free [NBP3-11681]

Immunoprecipitation: IL-33 Antibody (IL33305B) - Azide and BSA Free [NBP3-11681]

Immunoprecipitation: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunoprecipitation of recombinant hIL-33 proteins using IL-33 antibody (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.

Applications for IL-33 Antibody (IL33305B) - Azide and BSA Free

Application
Recommended Usage

Direct ELISA

1:2000-1:5000

ELISA

1:2000-1:5000

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:2000-1:5000
Application Notes
Western blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-33

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

Additional IL-33 Products

Product Documents for IL-33 Antibody (IL33305B) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-33 Antibody (IL33305B) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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