Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # I-1A4

Concentration

1.0 mg/ml

View all IL12B Antibodies »

Product Summary for IL12B Antibody (I-1A4)

Immunogen

Recombinant human IL-12

Specificity

Recognizes the p40 subunit of human interleukin-12, and binds to both the free subunit and the p35/p40 heterodimer. The reacts with both stimulated and unstimulated monocytes in immunohistology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IL12B Antibody (I-1A4)

ELISA: IL12B Antibody (I-1A4) - BSA Free [NB100-64693]

ELISA: IL12B Antibody (I-1A4) - BSA Free [NB100-64693]

ELISA: IL12B Antibody (I-1A4) [NB100-64693] - Direct ELISA showing cross reactivity of Mouse anti Human IL-12 with porcine IL-12. Antigen coated at 3ugml in ELISA coating buffer (BUF030) and Goat anti Mouse Ig (STAR87P) as tertiary detection reagent

Applications for IL12B Antibody (I-1A4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL12B

IL12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL12 is a potent stimulator of NK cells. IL12 therapy has been suggested as a method of enhancing cytocidal anti tumor immune responses.

Alternate Names

CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12BNK cell stimulatory factor chain 2, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40), interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NKSF, NKSF2IL12, subunit p40

Entrez Gene IDs

3593 (Human)

Gene Symbol

IL12B

UniProt

P29460

Additional IL12B Products

Product Documents for IL12B Antibody (I-1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL12B Antibody (I-1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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