IL6R Antibody (B-R6)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64770

Clone B-R6 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-64770
NB100-64770-0.025mg
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Conjugate
Catalog #
Alexa Fluor 350
NB100-64770AF350
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Alexa Fluor 405
NB100-64770AF405
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Alexa Fluor 488
NB100-64770AF488
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Alexa Fluor 532
NB100-64770AF532
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Alexa Fluor 594
NB100-64770AF594
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Alexa Fluor 647
NB100-64770AF647
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Alexa Fluor 700
NB100-64770AF700
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Alexa Fluor 750
NB100-64770AF750
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Allophycocyanin
NB100-64770APC
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Allophycocyanin/Cy7
NB100-64770APCCY7
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Biotin
NB100-64770B
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CoraFluor 1
NB100-64770CL1
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DyLight 350
NB100-64770UV
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DyLight 405
NB100-64770V
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DyLight 488
NB100-64770G
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DyLight 550
NB100-64770R
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DyLight 594
NB100-64770DL594
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DyLight 650
NB100-64770C
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DyLight 680
NB100-64770FR
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DyLight 755
NB100-64770IR
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FITC
NB100-64770F
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HRP
NB100-64770H
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Janelia Fluor 525
NB100-64770JF525
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Janelia Fluor 549
NB100-64770JF549
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Janelia Fluor 585
NB100-64770JF585
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Janelia Fluor 635
NB100-64770JF635
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Janelia Fluor 646
NB100-64770JF646
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Janelia Fluor 669
NB100-64770JF669
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mFluor Violet 450 SE
NB100-64770MFV450
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mFluor Violet 500 SE
NB100-64770MFV500
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mFluor Violet 610 SE
NB100-64770MFV610
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PE
NB100-64770PE
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PE/Atto594
NB100-64770PEATT594
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PE/Cy5.5
NB100-64770PECY55
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PE/Cy7
NB100-64770PECY7
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PerCP
NB100-64770PCP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B-R6

Concentration

1 mg/ml

View all IL6R Antibodies »

Product Summary for IL6R Antibody (B-R6)

Immunogen

CHO cells transfected with cDNA of the IL-6 receptor

Specificity

Recognizes soluble and membranous human IL-6R. This has been shown to inhibit IL-6 mediated proliferation of XG-1 cells, and partially blocks binding of IL-6 to its receptor. Removal of Sodium azide is recommended prior to use in functional assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IL6R Antibody (B-R6)

Flow Cytometry: IL6R Antibody (B-R6) - BSA Free [NB100-64770]

Flow Cytometry: IL6R Antibody (B-R6) - BSA Free [NB100-64770]

Flow Cytometry: IL6R Antibody (B-R6) [NB100-64770] - A surface stain was performed on human peripheral blood lymphocytes with IL6R (B-R6) antibody NB100-64770 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). A co-stain was performed with NBP2-24867AF488.

Applications for IL6R Antibody (B-R6)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL6R

IL6 exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa IL6 binding receptor protein (IL6R) and a 130 kDa signal transducing protein consisting of a 19 amino acid residue signal peptide (gp130). Human IL6R is a 468 amino acid residue type I membrane protein consisting of a 19 amino acid residue signal peptide, a 339 residue extracellular region, a 28 residue transmembrane region and an 82 residue cytoplasmic region. The ectodomain of IL6R is made up of an immunoglobulin like domain and a cytokine receptor superfamily domain. A naturally occurring soluble form of the IL6R, which binds IL6 and which mediates IL6 signaling through interaction with gp130, is identified in the urine of healthy adult humans. It has also been detected in human and mouse serum as well as in the conditioned medium of various cells including human peripheral blood mononuclear cells, and several T cell or granulocyte/macrophage cell lines. Increased expression of the c-fos and c-myc protooncogenes have been linked to IL6 receptor activation.

Alternate Names

CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991

Entrez Gene IDs

3570 (Human)

Gene Symbol

IL6R

UniProt

P08887

Additional IL6R Products

Product Documents for IL6R Antibody (B-R6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL6R Antibody (B-R6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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