IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12009

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # D7715A7

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IL6R Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising Lewis rats with OKT4 cells.

Specificity

This antibody is specific for murine IL6R.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009]

Immunocytochemistry/ Immunofluorescence: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009]

Immunocytochemistry/Immunofluorescence: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (NBP3-12009) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12009, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009]

Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009]

Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (NBP3-12009, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL6R

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Alternate Names

CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991

Gene Symbol

IL6R

Additional IL6R Products

Product Documents for IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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