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ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11729

Novus Biologicals, part of Bio-Techne
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NBP2-11729
NBP2-11729-0.025mg

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MM0413-9S32

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant ILT5/CD85a/LILRB3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free

Immunohistochemistry-Paraffin: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free [NBP2-11729]

Immunohistochemistry-Paraffin: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free [NBP2-11729]

Immunohistochemistry-Paraffin: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) [NBP2-11729] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-11729. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.
Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free [NBP2-11729]

Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free [NBP2-11729]

Flow Cytometry: ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) [NBP2-11729] - Analysis using Allophycocyanin conjugate of NBP2-11729. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.

Applications for ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LILRB3/CD85a/ILT5

LILRB3 is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signaling modulators and inhibitors of cell activation. LILRB3 may act as receptor for class I MHC antigens.

Alternate Names

CD85a, ILT5, LIR3

Gene Symbol

LILRB3

UniProt

Additional LILRB3/CD85a/ILT5 Products

Product Documents for ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ILT5/CD85a/LILRB3 Antibody (MM0413-9S32) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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