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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 270 and 320 of human imprinted and ancient homolog using the numbering given in entry NP_060909.1 (GeneID 55364).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IMPACT Antibody

Western Blot: IMPACT Antibody [NBP1-42678]

Western Blot: IMPACT Antibody [NBP1-42678]

Western Blot: IMPACT Antibody [NBP1-42678] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IMPACT antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. IMPACT was also immunoprecipitated by another rabbit anti-IMPACT antibody which recognizes an upstream epitope.

Applications for IMPACT Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IMPACT

Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis

Alternate Names

Impact homolog (mouse), Imprinted and ancient gene protein homolog, MGC33718, protein IMPACT

Entrez Gene IDs

55364 (Human)

Gene Symbol

IMPACT

Additional IMPACT Products

Product Documents for IMPACT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IMPACT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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