IMPACT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86221
![Western Blot: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Western-Blot-NBP1-86221-img0008.jpg "Western Blot: IMPACT Antibody [NBP1-86221]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (98%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ENKKIASATHNIYAYRIYCEDKQTFLQDCEDDGETAAGGRLLHLMEILNVKNVMVVV
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30466445).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IMPACT Antibody
![Western Blot: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Western-Blot-NBP1-86221-img0012.jpg "Western Blot: IMPACT Antibody [NBP1-86221]")
Western Blot: IMPACT Antibody [NBP1-86221]
Western Blot: IMPACT Antibody [NBP1-86221] - IMPACT overexpression confers GL261 glioma cells increased survival during tryptophan deprivation. Western blotting with indicated antibodies on GL261-wild-type and GL261-IMPACThigh lines cultured in standard media containing 50 uM tryptophan over a period of 8 days. Doubling time is shown as a mean +/- SD. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-018-0301-7), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86221-img0007.jpg "Immunocytochemistry/ Immunofluorescence: IMPACT Antibody [NBP1-86221]")
Immunocytochemistry/ Immunofluorescence: IMPACT Antibody [NBP1-86221]
Immunocytochemistry/Immunofluorescence: IMPACT Antibody [NBP1-86221] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Immunohistochemistry-Paraffin-NBP1-86221-img0010.jpg "Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]")
Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]
Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Immunohistochemistry-Paraffin-NBP1-86221-img0009.jpg "Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]")
![Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]](https://resources.bio-techne.com/images/products/IMPACT-Antibody-Immunohistochemistry-Paraffin-NBP1-86221-img0011.jpg "Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]")
Applications for IMPACT Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IMPACT
Alternate Names
Impact homolog (mouse), Imprinted and ancient gene protein homolog, MGC33718, protein IMPACT
Gene Symbol
IMPACT
Additional IMPACT Products
Product Documents for IMPACT Antibody
Product Specific Notices for IMPACT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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