Importin-7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46168

Novus Biologicals, part of Bio-Techne
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NBP1-46168
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Importin-7 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human Importin 7 using the numbering given in entry NP_006382.1 (GeneID 10527).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Importin-7 Antibody

Western Blot: Importin-7 Antibody [NBP1-46168]

Western Blot: Importin-7 Antibody [NBP1-46168]

Western Blot: Importin-7 Antibody [NBP1-46168] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Importin 7 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody NBP1-46167, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: Importin-7 Antibody [NBP1-46168]

Immunohistochemistry-Paraffin: Importin-7 Antibody [NBP1-46168]

Immunohistochemistry-Paraffin: Importin-7 Antibody [NBP1-46168] - Section of human colon carcinoma. Antibody: Affinity purified rabbit antiImportin 7 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB

Applications for Importin-7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Importin-7

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]

Alternate Names

FLJ14581, Imp7MGC138673, importin 7, RAN binding protein 7, Ran-binding protein 7, RanBP7, RANBP7importin-7

Entrez Gene IDs

10527 (Human)

Gene Symbol

IPO7

Additional Importin-7 Products

Product Documents for Importin-7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Importin-7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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