Importin-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24751

Novus Biologicals, part of Bio-Techne
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NBP2-24751
NBP2-24751SS
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Equine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Importin-8 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 850-900 of human Importin-8 was used as immunogen for the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Importin-8 Antibody

Western Blot: Importin-8 Antibody [NBP2-24751]

Western Blot: Importin-8 Antibody [NBP2-24751]

Western Blot: Importin-8 Antibody [NBP2-24751] - Analysis of Importin-8 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using Importin-8 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody [NBP2-24751]

Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody [NBP2-24751]

Immunocytochemistry/Immunofluorescence: Importin-8 Antibody [NBP2-24751] - ICC staining of HeLa cells using Importin-8 antibody at 5 ug/ml.
Simple Western: Importin-8 Antibody [NBP2-24751]

Simple Western: Importin-8 Antibody [NBP2-24751]

Simple Western: Importin-8 Antibody [NBP2-24751] - Simple Western lane view shows a specific band for Importin-8 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Importin-8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:2000

Immunoprecipitation

reported in scientific literature (PMID 19131589)

Simple Western

1:20

Western Blot

1:200-1:2000
Application Notes
Alternative initiation produces three different forms of this protein (approximately 47, 29 and 27 kD). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin-8

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. [provided by RefSeq]

Alternate Names

FLJ21954, FLJ26580, IMP8, importin 8, importin-8, RAN binding protein 8, Ran-binding protein 8, RanBP8, RANBP8imp8

Gene Symbol

IPO8

UniProt

O15397 (Human)

Additional Importin-8 Products

Product Documents for Importin-8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Importin-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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