Importin beta/KPNB1 Antibody (PSH01-59)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32510

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32510
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-59

Concentration

1 mg/ml

View all Importin beta/KPNB1 Antibodies »

Product Summary for Importin beta/KPNB1 Antibody (PSH01-59)

Immunogen

Recombinant protein within human Importin beta/KPNB1 aa 1-550. (Uniprot: Q14974)

Localization

Cytoplasm. Nucleus envelope.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Importin beta/KPNB1 Antibody (PSH01-59)

Importin beta/KPNB1 Antibody (PSH01-59)

Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510]

Immunoprecipitation: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510] - Importin beta/KPNB1 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32510 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32510 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit IgG instead of NBP3-32510 in HeLa cell lysate
Lane 3: NBP3-32510 IP in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
Importin beta/KPNB1 Antibody (PSH01-59)

Western Blot: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510]

Western Blot: Importin beta/KPNB1 Antibody (PSH01-59) [NBP3-32510] - Western blot analysis of Importin beta/KPNB1 on different lysates with Rabbit anti-Importin beta/KPNB1 antibody (NBP3-32510) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: A431 cell lysate (20 ug/Lane)
Lane 4: A549 cell lysate (20 ug/Lane)
Lane 5: Jurkat cell lysate (20 ug/Lane)
Lane 6: K-562 cell lysate (20 ug/Lane)
Lane 7: Caco-2 cell lysate (20 ug/Lane)
Lane 8: NIH/3T3 cell lysate (20 ug/Lane)
Lane 9: PC-12 cell lysate (20 ug/Lane)
Lane 10: C6 cell lysate (20 ug/Lane)
Lane 11: Mouse spleen tissue lysate (40 ug/Lane)
Lane 12: Mouse testis tissue lysate (40 ug/Lane)
Lane 13: Rat spleen tissue lysate (40 ug/Lane)
Lane 14: Rat testis tissue lysate (40 ug/Lane)

Predicted band size: 97 kDa
Observed band size: 97 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Applications for Importin beta/KPNB1 Antibody (PSH01-59)

Application
Recommended Usage

Immunoprecipitation

1-2ug/sample

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Importin beta/KPNB1

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq]

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Gene Symbol

KPNB1

Additional Importin beta/KPNB1 Products

Product Documents for Importin beta/KPNB1 Antibody (PSH01-59)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Importin beta/KPNB1 Antibody (PSH01-59)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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