Importin beta/KPNB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40784

Novus Biologicals, part of Bio-Techne
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NB100-40784
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Importin beta/KPNB1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RRSKTNKAKTLAT corresponding to C-Terminus according to NP_002256.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Importin beta/KPNB1 Antibody

Western Blot: Importin beta/KPNB1 Antibody [NB100-40784]

Western Blot: Importin beta/KPNB1 Antibody [NB100-40784]

Western Blot: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of A431 (A), HEK293 (B), Jurkat (C) cell lysates with antibody at 0.03 ug/mL, Daudi (D), HeLa (E) cell lysates with antibody at 0.01 ug/mL, and Kelly (F) and KNRK (G) cell lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784]

Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784]

Immunocytochemistry/Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784]

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784]

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of paraffin embedded Human Spleen with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Importin beta/KPNB1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin beta/KPNB1

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Entrez Gene IDs

3837 (Human); 16211 (Mouse); 24917 (Rat)

Gene Symbol

KPNB1

UniProt

NP_002256.2 (Human)

Additional Importin beta/KPNB1 Products

Product Documents for Importin beta/KPNB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Importin beta/KPNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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