Importin beta/KPNB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79806

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Importin beta/KPNB1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 825 and the C-terminus (residue 876) of human Karyopherin (importin) beta 1 using the numbering given in entry NP_002256.2 (GeneID 3837).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Importin beta/KPNB1 Antibody

Western Blot: Importin beta/KPNB1 Antibody [NB100-79806]

Western Blot: Importin beta/KPNB1 Antibody [NB100-79806]

Western Blot: Importin beta/KPNB1 Antibody [NB100-79806] - Importin beta Antibody [NB100-79806] - Detection of KapB1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-KapB1 antibody BL1964 (Cat. No. NB100-79806) used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was unsuccessful when using rabbit anti-KapB1 antibody BL1962 (Cat. No. NB100-79805) at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).

Applications for Importin beta/KPNB1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:5000-1:15000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Importin beta/KPNB1

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Entrez Gene IDs

3837 (Human); 16211 (Mouse)

Gene Symbol

KPNB1

UniProt

NP_002256.2 (Human)

Additional Importin beta/KPNB1 Products

Product Documents for Importin beta/KPNB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Importin beta/KPNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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