Importin beta/KPNB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87712

Novus Biologicals, part of Bio-Techne
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NBP1-87712
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Importin beta/KPNB1 Antibodies »

Product Summary for Importin beta/KPNB1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Importin beta/KPNB1 Antibody

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712]

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712]

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining in human testis and skeletal muscle tissues . Corresponding KPNB1 RNA-seq data are presented for the same tissues.
Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712]

Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712]

Western Blot: Importin beta/KPNB1 Antibody [NBP1-87712] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NBP1-87712]

Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NBP1-87712]

Immunocytochemistry/Immunofluorescence: Importin beta/KPNB1 Antibody [NBP1-87712] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol. Antibody staining is shown in green.
View 5 more images

Applications for Importin beta/KPNB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Importin beta/KPNB1

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq]

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Entrez Gene IDs

3837 (Human)

Gene Symbol

KPNB1

UniProt

Q14974 (Human)

Additional Importin beta/KPNB1 Products

Product Documents for Importin beta/KPNB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Importin beta/KPNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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