Indian Hedgehog/Ihh Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59443

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Indian Hedgehog/Ihh Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to IHH(Indian hedgehog homolog (Drosophila)) The peptide sequence was selected from the N terminal of IHH. Peptide sequence AAWGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIAR. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33867837).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Indian Hedgehog/Ihh Antibody

Western Blot: Indian Hedgehog/Ihh Antibody [NBP1-59443]

Western Blot: Indian Hedgehog/Ihh Antibody [NBP1-59443]

Western Blot: Indian Hedgehog/Ihh Antibody [NBP1-59443] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml
Immunohistochemistry-Paraffin: Indian Hedgehog/Ihh Antibody [NBP1-59443]

Immunohistochemistry-Paraffin: Indian Hedgehog/Ihh Antibody [NBP1-59443]

Immunohistochemistry-Paraffin: Indian Hedgehog/Ihh Antibody [NBP1-59443] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Applications for Indian Hedgehog/Ihh Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Indian Hedgehog/Ihh

IHH is an intercellular signal essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. It is implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones and induces the expression of parathyroid hormone-related protein (PTHRP).

Alternate Names

BDA1, HHG2, Ihh

Gene Symbol

IHH

UniProt

Q14623 (Human)

Additional Indian Hedgehog/Ihh Products

Product Documents for Indian Hedgehog/Ihh Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Indian Hedgehog/Ihh Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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