Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76408

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76408
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR040F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Indoleamine 2,3-dioxygenase/IDO Antibodies »

Product Summary for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

Immunogen

Recombinant protein representing Human IDO1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR040F] (NBP2-76408). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Vinculin.
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F) [NBP2-76408] - Detection of human IDO1 by WB of immunoprecipitates from HeLa lysate treated with IFN-gamma. Antibodies: Rabbit anti-IDO1 recombinant monoclonal [BLR040F] (NBP2-76408) and rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404).

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

Application
Recommended Usage

Immunoprecipitation

15 ul/1mg lysate

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR040F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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