Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46020

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B5

Concentration

1 mg/ml

View all Indoleamine 2,3-dioxygenase/IDO Antibodies »

Product Summary for Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

Immunogen

Full length human recombinant protein of human IDO1(NP_002155) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of extracts (10ug) from 2 different cell lines by using anti-IDO1 monoclonal antibody at 1:200.
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5) [NBP2-46020]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Staining of paraffin-embedded Human lymphoma tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.
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Applications for Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000
Application Notes
WB application notes: for overexpressing samples up to a 1:4000 dilution can be used. For detecting endogenous protein, a starting dilution of 1:200 will be necessary.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Indoleamine 2,3-dioxygenase/IDO

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody (OTI2B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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