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Indoleamine 2,3-dioxygenase/IDO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80106

Novus Biologicals, part of Bio-Techne
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NBP2-80106

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Summary for Indoleamine 2,3-dioxygenase/IDO Antibody

Immunogen

Recombinant mouse Indoleamine 2,3-dioxygenase/IDO.

Specificity

Recognizes mouse Indoleamine 2,3-dioxygenase/IDO. Detects a band of ~45kDa by Western blot.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106] - analysis of mouse IDO using anti-IDO (mouse), pAb at 1:2,000 dilution. 1. Recombinant mouse IDO (His-tagged). 2. Con-A treated mouse peripheral blood lymphocyte lysate.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106] - Staining of mouse gut using anti-IDO (mouse), pAb. Colorization by ABS Reagent (Vectastain) and counterstaining with hematoxylin.

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1:2000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Indoleamine 2,3-dioxygenase/IDO

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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