Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933)

Name: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933)
Brand: Novus Biologicals
SKU: NBP3-13493

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13493

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Virus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # GT1236

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Recombinant protein of Influenza A H1N1 Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Specificity

Influenza A H1N1 Nucleoprotein. (A/WSN/1933)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933)

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236)(A/WSN/1933) [NBP3-13493]

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by Western blot analysis. A. 5 ug DF1 whole cell lysate/extract B. 5 ug whole cell lysate/extract of WSN virus infected DF1 cells (10 hr) 7.5 % SDS-PAGE Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) dilution: 1:5000

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493]

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by immunofluorescent analysis. Sample: DF1 cells infected with Influenza A virus (WSN) . Green: Influenza A H1N1 Nucleoprotein stained by Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:500. Blue: Hoechst 33342 staining.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

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