Influenza A nucleoprotein Antibody (010)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12741

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Influenza A Virus H3N2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 010

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Influenza A nucleoprotein Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H3N2 (Uniprot#: P22435.2; Met1-Asn498).

Reactivity Notes

Pan Influenza A

Has cross-reactivity in ELISA with:
H1N1 (A/Brevig Mission/1/1918) NP
H1N1 (A/Brisbane/02/2018) NP
H1N1 (A/California/07/2009) NP
H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP
H1N1 (A/Hawaii/70/2019) NP
H1N1 (A/Michigan/45/2015 NP
H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M)
H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP
H2N2 (A/Ann Arbor/6/1960) NP
H3N2 (A/Aichi/2/1968) NP
H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP
H3N2 (A/Hong Kong/1/1968) NP
H3N2 (A/Hong Kong/2671/2019) NP
H3N2 (A/Hong Kong/45/2019) NP
H3N2 (A/Hong Kong/4801/2014) NP
H3N2 (A/Kansas/14/2017) NP
H3N2 (A/Switzerland/9715293/2013) NP
H7N9 (A/Anhui/1-BALF_RG6/2013) NP
H7N9 (A/Shanghai/2/2013) NP
Influenza B (B/Phuket/3073/2013) NP
Influenza B (B/Brisbane/60/2008) NP
Influenza B (B/Colorado/06/2017) NP

No cross-reactivity in ELISA with:
Influenza B (B/Florida/4/2006) NP
Influenza B (B/Washington/02/2019) NP
SARS-CoV-2 Nucleocapsid Protein
HCoV-229E Nucleocapsid Protein
HCoV-NL63 Nucleocapsid Protein
HCoV-HKU1 Nucleocapsid Protein
HCoV-OC43 Nucleocapsid Protein

Specificity

Widely recognize Influenza A Nucleoprotein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Influenza A nucleoprotein Antibody (010)

Western Blot: Influenza A nucleoprotein Antibody (010) [NBP3-12741]

Western Blot: Influenza A nucleoprotein Antibody (010) [NBP3-12741]

Western Blot: Influenza A nucleoprotein Antibody (010) [NBP3-12741] - Reocombinant Protein 10 ng
Influenza A nucleoprotein Antibody (010) Western Blot: Influenza A nucleoprotein Antibody (010) [NBP3-12741]

Western Blot: Influenza A nucleoprotein Antibody (010) [NBP3-12741]

NBP3-12741 used at 1 ug/mL. Lane A: H3N2 (A/Darwin/9/2021)/(A/Darwin/6/2021) NP Protein. Lane B: Influenza B (B/Austria/1359417/2021) NP Protein. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.

Applications for Influenza A nucleoprotein Antibody (010)

Application
Recommended Usage

ELISA

0.1-0.2 ug/mL

Western Blot

0.1-1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A nucleoprotein

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Alternate Names

Influenza A virus NP, NP, NP-A, Nucleocapsid protein, Nucleoprotein

Additional Influenza A nucleoprotein Products

Product Documents for Influenza A nucleoprotein Antibody (010)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Influenza A nucleoprotein Antibody (010)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.