iNOS Antibody (BLR181J)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14750
Recombinant Monoclonal
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR181J
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
between 1094 and 1144 (C-term)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for iNOS Antibody (BLR181J)
Western Blot;iNOS Antibody (BLR181J)[NBP3-14750]
Western Blot;iNOS Antibody (BLR181J) Whole cell lysate (10 µg) from RAW 264.7 and LPS-treated RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .Applications for iNOS Antibody (BLR181J)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: iNOS
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
References
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
Long Name
Inducible Nitic Oxide Synthase
Alternate Names
NOS2, NOS2A
Gene Symbol
NOS2
Additional iNOS Products
Product Documents for iNOS Antibody (BLR181J)
Product Specific Notices for iNOS Antibody (BLR181J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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