iNOS Antibody (JE59-61)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32472
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE59-61
Concentration
1 mg/ml
Product Summary for iNOS Antibody (JE59-61)
Immunogen
Recombinant protein within Human iNOS aa 1-300 / 1,153. (Uniprot: P35228)
Localization
Cytoplasm, cytosol.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for iNOS Antibody (JE59-61)
Western Blot: iNOS Antibody (JE59-61) [NBP3-32472]
Western Blot: iNOS Antibody (JE59-61) [NBP3-32472] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32472) at 1/1,000 dilution.Lane 1: RAW264.7 whole cell lysate
Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours whole cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for iNOS Antibody (JE59-61)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: iNOS
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
References
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
Long Name
Inducible Nitic Oxide Synthase
Alternate Names
NOS2, NOS2A
Gene Symbol
NOS2
Additional iNOS Products
Product Documents for iNOS Antibody (JE59-61)
Product Specific Notices for iNOS Antibody (JE59-61)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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