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INPP4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85883

Novus Biologicals, part of Bio-Techne
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NBP1-85883
NBP1-85883-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QDSIPHHSDYDEEEWDRVWANVGKSLNCIIAMVDKLIERDGGSEGSGGNNDGEKEPSLTDAIPSHPREDWYEQLYPLILTLKDCMGEVVNRAKQ

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25277204). Mouse (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for INPP4B Antibody

Western Blot: INPP4B Antibody [NBP1-85883]

Western Blot: INPP4B Antibody [NBP1-85883]

Western Blot: INPP4B Antibody [NBP1-85883] - Analysis in human cell lines MCF-7 and HEK293 using Anti-INPP4B antibody. Corresponding INPP4B RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: INPP4B Antibody [NBP1-85883]

Immunocytochemistry/ Immunofluorescence: INPP4B Antibody [NBP1-85883]

Immunocytochemistry/Immunofluorescence: INPP4B Antibody [NBP1-85883] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: INPP4B Antibody [NBP1-85883]

Immunohistochemistry-Paraffin: INPP4B Antibody [NBP1-85883]

Immunohistochemistry-Paraffin: INPP4B Antibody [NBP1-85883] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Applications for INPP4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25277204). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: INPP4B

INPP4B encodes the inositol polyphosphate 4-phosphatase type II, one of the enzymes involved in phosphatidylinositolsignaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol3,4-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing,as has been established for the type I enzyme. (provided by RefSeq)

Alternate Names

inositol polyphosphate-4-phosphatase, type II, 105kDa, type II inositol-3,4-bisphosphate 4-phosphatase

Gene Symbol

INPP4B

Additional INPP4B Products

Product Documents for INPP4B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for INPP4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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