Insulin Antibody (IRDN/1980R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07511
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Insulin Antibody (IRDN/1980R) [NBP3-07511]](https://resources.bio-techne.com/images/products/Insulin-Antibody-IRDN-1980R-Immunohistochemistry-Paraffin-NBP3-07511-img0001.jpg "Immunohistochemistry-Paraffin: Insulin Antibody (IRDN/1980R) [NBP3-07511]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Porcine, Bovine, Rabbit
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # IRDN/1980R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length Insulin protein (IRDN) (Uniprot: P01308)
Localization
Cytoplasmic
Specificity
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Marker
beta-Cell & Insulinoma Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08836)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Insulin Antibody (IRDN/1980R)
Immunohistochemistry-Paraffin: Insulin Antibody (IRDN/1980R) [NBP3-07511]
Immunohistochemistry-Paraffin: Insulin Antibody (IRDN/1980R) [NBP3-07511] - Formalin-fixed, paraffin-embedded human Pancreas stained with Insulin Rabbit Recombinant Monoclonal Antibody (IRDN/1980R).Applications for Insulin Antibody (IRDN/1980R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Insulin
Additional Insulin Products
Product Documents for Insulin Antibody (IRDN/1980R)
Product Specific Notices for Insulin Antibody (IRDN/1980R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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