Insulin Antibody (SPM139)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32974

Novus Biologicals, part of Bio-Techne
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NBP2-32974-0.02mg
NBP2-32974-0.1mg
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Key Product Details

Species Reactivity

Human, Porcine, Bovine, Mouse (Negative), Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM139

Concentration

0.2 mg/ml

View all Insulin Antibodies »

Product Specifications

Immunogen

Full length (1-84 amino acid) Purified pig insulin, conjugated to KLH

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Cytoplasmic

Specificity

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Marker

beta-Cell & Insulinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34738)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Insulin Antibody (SPM139)

Immunohistochemistry-Paraffin: Insulin Antibody (SPM139) [NBP2-32974]

Immunohistochemistry-Paraffin: Insulin Antibody (SPM139) [NBP2-32974]

Immunohistochemistry-Paraffin: Insulin Antibody (SPM139) [NBP2-32974] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monclonal Antibody.

Applications for Insulin Antibody (SPM139)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

UniProt

P01308, P01317 (Bovine)

Additional Insulin Products

Product Documents for Insulin Antibody (SPM139)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Insulin Antibody (SPM139)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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