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Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # D3E7cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human insulin.

Specificity

This is specific for both insulin and proinsulin. Cross-reacts with human, bovine and porcine insulin and proinsulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Insulin/Proinsulin Antibody (D3E7cc)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Kd for D3E7cc is 6.3 x 10^-8 M. Detection of rat-mouse insulin/proinsulin in two-site enzyme immunoassay. Recommend pair (capture - detection): D6C4cc (NB120-8304) - D3E7cc (NB200-435).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Insulin/Proinsulin

Detection of insulin and proinsulin

Alternate Names

IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin

Entrez Gene IDs

3630 (Human); 24505 (Rat)

Gene Symbol

INS

Additional Insulin/Proinsulin Products

Product Documents for Insulin/Proinsulin Antibody (D3E7cc)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Insulin/Proinsulin Antibody (D3E7cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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