Insulysin/IDE Antibody (GT143)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13560

Novus Biologicals, part of Bio-Techne
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NBP3-13560
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Canine (99%), Rhesus Macaque (100%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT143

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Insulysin/IDE Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Insulysin/IDE. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Insulysin/IDE Antibody (GT143)

Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Wild-type (WT) and Insulysin/IDE knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Immunohistochemistry-Paraffin: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Insulysin/IDE antibody [GT143] detects Insulysin/IDE protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560]

Western Blot: Insulysin/IDE Antibody (GT143) [NBP3-13560] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Insulysin/IDE antibody [GT143] (NBP3-13560) diluted at 1:1000.
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Applications for Insulysin/IDE Antibody (GT143)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Insulysin/IDE

Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer's disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.

Long Name

Insulin Degrading Enzyme

Alternate Names

IDE, INSDEGM, Insulinase

Gene Symbol

IDE

Additional Insulysin/IDE Products

Product Documents for Insulysin/IDE Antibody (GT143)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Insulysin/IDE Antibody (GT143)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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