Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 31H4

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha 2/CD49b Antibodies »

Product Summary for Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

Immunogen

Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.

Specificity

It is specific to the alpha 2 subunit by relative expression of antigen on various cell lines by Flow Cytomery, and its recognition of affinity purified alpha 2 beta 1 in Dot Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500
Application Notes
Capable of immunoprecipitating the Integrin alpha 2/CD49b subunit from human cell-lines surface labelled with 125I. It has been confirmed specific to Integrin alpha 2/CD49b by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified Integrin alpha 2 beta 1 in dot blots.This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 2/CD49b

Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Gene Symbol

ITGA2

Additional Integrin alpha 2/CD49b Products

Product Documents for Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha 2/CD49b Antibody (31H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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