Flow Cytometry: Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin] [NBP1-43649] - Staining of mouse platelets with Anti-Mouse/Rat CD61 (Integrin beta 3) FITC and 0.06 ug of Rat IgG1 K Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse CD41 Biotin (right) followed by Streptavidin PE.

Applications for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

This MWReg30 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin alpha 2b/CD41

The MWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alphaIIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin betaIII) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin. Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Gene Symbol

ITGA2B

Additional Integrin alpha 2b/CD41 Products

Product Documents for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43649

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

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Product Summary for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Applications for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin alpha 2b/CD41

Alternate Names

Gene Symbol

Additional Integrin alpha 2b/CD41 Products

Product Documents for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Integrin alpha 2b/CD41 Antibody (MWReg30) [Biotin]

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