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Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09130

Novus Biologicals, part of Bio-Techne
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NBP3-09130

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 5H10-27 (MFR5)

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

C57BL/6 x A/J)F1 murine mast cell line

Specificity

The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) [NBP3-09130] -

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) [NBP3-09130] - Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 ug/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).
Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) -

Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - Flow cytometry surface staining pattern of murine bone marrow using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC).

Applications for Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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