Integrin alpha 5/CD49e Antibody (JJ08-94)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67883

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ08-94

Concentration

1 mg/ml

View all Integrin alpha 5/CD49e Antibodies »

Product Specifications

Immunogen

Synthetic peptide within C-terminal human Integrin alpha 5/CD49e. (SwissProt: P08648 Human; SwissProt: P11688 Mouse; Unigene:100796 Rat)

Localization

Membrane, Cell junction, Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha 5/CD49e Antibody (JJ08-94)

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2
Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883]

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.
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Applications for Integrin alpha 5/CD49e Antibody (JJ08-94)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 5/CD49e

CD49e is a type I integral membrane glycoprotein, known as alpha5 integrin, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Gene Symbol

ITGA5

Additional Integrin alpha 5/CD49e Products

Product Documents for Integrin alpha 5/CD49e Antibody (JJ08-94)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 5/CD49e Antibody (JJ08-94)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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