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Integrin alpha 9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16972

Novus Biologicals, part of Bio-Techne
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NBP2-16972

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Mouse (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Integrin alpha 9. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%).

Localization

Membrane, Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha 9 Antibody

Western Blot: Integrin alpha 9 Antibody [NBP2-16972]

Western Blot: Integrin alpha 9 Antibody [NBP2-16972]

Western Blot: Integrin alpha 9 Antibody [NBP2-16972] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 9 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972]

Immunocytochemistry/ Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972]

Immunocytochemistry/Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972] - Cultured human keratinocytes plated on fibronectin coated coverslips. Anti integrin alpha 9 antibody diluted 1:50 in 5% BSA, incubated at room temperature for 3 hours. Nuclei stained with DAPI (green) and f-actin with phalloidin (purple). ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Integrin alpha 9 Antibody [NBP2-16972]

Immunohistochemistry-Paraffin: Integrin alpha 9 Antibody [NBP2-16972]

Immunohistochemistry-Paraffin: Integrin alpha 9 Antibody [NBP2-16972] - Human hepatoma, using Integrin alpha 9 antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Applications for Integrin alpha 9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-16972 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Integrin alpha 9

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq]

Alternate Names

ITGA4L, ITGA9, RLC-alpha

Gene Symbol

ITGA9

UniProt

Additional Integrin alpha 9 Products

Product Documents for Integrin alpha 9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha 9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.